19946275258
| Catalog Number | PME100487 |
|---|---|
| Package | 10 μg ￥600.00 ; 50 μg ￥2450.00 ; 100 μg ￥3680.00 |
| Description | Recombinant SARS-CoV-2 (2019-nCoV) S protein RBD protein with C-terminal human Fc tag |
| Synonyms | S protein RBD, Spike glycoprotein Receptor-binding domain, S glycoprotein RBD, Spike protein RBD, COVID-19 |
| Delivery | In Stock |
| Uniprot ID | P0DTC2 |
| Expression Host | HEK293 |
| Tag | C-Human Fc tag |
| Molecular Characterization |  |
| Molecular Weight | The protein has a predicted molecular mass of 51.2 kDa after removal of the signal peptide. |
| Purity | The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
| Formulation & Reconstitution | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions. |
| Storage | Store at -80°C for 12 months (Avoid repeated freezing and thawing) |
| Usage | Research use only |
| Images |
Figure 1. SARS-CoV-2 (2019-nCoV) S protein RBD, hFc Tag on SDS-PAGE under reducing condition.
Figure 2. ELISA plate pre-coated by 2 μg/ml (100 μl/well) S-RBD, hFc tagged protein (PME100487) can bind Human ACE2, mFc Tagged protein（PME100072） in a linear range of 0.976-39.35 ng/ml.
Figure 3. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human ACE2, His Tagged protein（PME100490）can bind S-RBD, hFc tagged protein (PME100487) in a linear range of 0.128-80.0 ng/ml. |
| Background | SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) also known as Covid19 (2019 Novel Coronavirus) is a virus that causes illnesses ranging from the common cold to severe diseases. The spike protein is a type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which accounts for recognizing the cell surface receptor, ACE2. S2 contains basic elements needed for the membrane fusion. Recent publications indicate that S1-RBD domain can induce virus neutralizing-antibody and T cell response. |
